Cytosolic non-specific dipeptidase (CNDP2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-475aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AALTTLFKYI DENQDRYIKK LAKWVAIQSV SAWPEKRGEI RRMMEVAAAD VKQLGGSVEL VDIGKQKLPD GSEIPLPPIL LGRLGSDPQK KTVCIYGHLD VQPAALEDGW DSEPFTLVER DGKLYGRGST DDKGPVAGWI NALEAYQKTG QEIPVNVRFC LEGMEESGSE GLDELIFARK DTFFKDVDYV CISDNYWLGK KKPCITYGLR GICYFFIEVE CSNKDLHSGV YGGSVHEAMT DLILLMGSLV DKRGNILIPG INEAVAAVTE EEHKLYDDID FDIEEFAKDV GAQILLHSHK KDILMHRWRY PSLSLHGIEG AFSGSGAKTV IPRKVVGKFS IRLVPNMTPE VVGEQVTSYL TKKFAELRSP NEFKVYMGHG GKPWVSDFSH PHYLAGRRAM KTVFGVEPDL TREGGSIPVT LTFQEATGKN VMLLPVGSAD DGAHSQNEKL NRYNYIEGTK MLAAYLYEVS QLKD. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Isoform 2 may be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. Reference: Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase.Teufel M., Saudek V., Ledig J.P., Bernhardt A., Boularand S., Carreau A., Cairns N.J., Carter C., Cowley D.J., Duverger D., Ganzhorn A.J., Guenet C., Heintzelmann B., Laucher V., Sauvage C., Smirnova T.J. Biol. Chem. 278:6521-6531(2003). Function: Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly