Cytochrome P450 3A16 (Cyp3a16), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-504aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MNLFSALSLD TLVLLAIILV LLYRYGTCTH GLFKKQGIPG PKPLPFLGTV LNYYKGLWKF DMECYEKYGK TWGLFDGQIP LFVITDPETI KNVLVKECFS VFTNRQDFFP VGIMSKSISL AKDEEWKRYR ALLSPTFTSG NLKEMFPVIE QYGDILVKYL RQEAEKGKPV AVKDVLGAYS MDVIISTTFG VNIDSLNNPE DPFVENAKKV LRFDYFDPLS LSVALFPFLT PIYEMLNICM FPKDSIEFFK KFVDRMTENR LDSKQKHRVD FIYLMMEAYN KSKDKDSHKA LSEIEITAQS IIFIFAGYET TSSILSFTVY SLATHPDIQK KLQEEIDEAL PNKAPPTYDT VMAMEYLDMV LNETLRLYPI TNRLQRVCKK DVEINGIYIP KGSTVIIPSY VLHHDPQHWP EPEEFQPERF SKENKGSIDP YVYLPFGNGP RNCIGMRFAL MNMKLALIKV LQNFSFQPCK ETQIPLKLSR ELLLQPVKPI VLKVVPRDAV ITGA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Reference: "Dynamic patterns of histone methylation are associated with ontogenic expression of the Cyp3a genes during mouse liver maturation." Li Y., Cui Y., Hart S.N., Klaassen C.D., Zhong X.B. Mol Pharmacol 75:1171-1179(2009). Function: nan