Cytochrome P450 2C8 (CYP2C8), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 27-490aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: RKLPPGPTPL PIIGNMLQID VKDICKSFTN FSKVYGPVFT VYFGMNPIVV FHGYEAVKEA LIDNGEEFSG RGNSPISQRI TKGLGIISSN GKRWKEIRRF SLTTLRNFGM GKRSIEDRVQ EEAHCLVEEL RKTKASPCDP TFILGCAPCN VICSVVFQKR FDYKDQNFLT LMKRFNENFR ILNSPWIQVC NNFPLLIDCF PGTHNKVLKN VALTRSYIRE KVKEHQASLD VNNPRDFIDC FLIKMEQEKD NQKSEFNIEN LVGTVADLFV AGTETTSTTL RYGLLLLLKH PEVTAKVQEE IDHVIGRHRS PCMQDRSHMP YTDAVVHEIQ RYSDLVPTGV PHAVTTDTKF RNYLIPKGTT IMALLTSVLH DDKEFPNPNI FDPGHFLDKN GNFKKSDYFM PFSAGKRICA GEGLARMELF LFLTTILQNF NLKSVDDLKN LNTTAVTKGI VSLPPSYQIC FIPV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. In the epoxidation of arachidonic acid it generates only 14,15- and 11,12-cis-epoxyeicosatrienoic acids. It is the principal enzyme responsible for the metabolism the anti-cancer drug paclitaxel. Reference: "cDNA and amino acid sequences of two members of the human P450IIC gene subfamily." Kimura S., Pastewka J., Gelboin H.V., Gonzalez F.J. Nucleic Acids Res. 15:10053-10054(1987). Function: nan