Cytochrome P450 2A6 (CYP2A6), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 29-494aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SKGKLPPGPT PLPFIGNYLQ LNTEQMYNSL MKISERYGPV FTIHLGPRRV VVLCGHDAVR EALVDQAEEF SGRGEQATFD WVFKGYGVVF SNGERAKQLR RFSIATLRDF GVGKRGIEER IQEEAGFLID ALRGTGGANI DPTFFLSRTV SNVISSIVFG DRFDYKDKEF LSLLRMMLGI FQFTSTSTGQ LYEMFSSVMK HLPGPQQQAF QLLQGLEDFI AKKVEHNQRT LDPNSPRDFI DSFLIRMQEE EKNPNTEFYL KNLVMTTLNL FIGGTETVST TLRYGFLLLM KHPEVEAKVH EEIDRVIGKN RQPKFEDRAK MPYMEAVIHE IQRFGDVIPM SLARRVKKDT KFRDFFLPKG TEVFPMLGSV LRDPSFFSNP QDFNPQHFLN EKGQFKKSDA FVPFSIGKRN CFGEGLARME LFLFFTTVMQ NFRLKSSQSP KDIDVSPKHV GFATIPRNYT MSFLPR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Acts as a 1,4-cineole 2-exo-monooxygenase. Possesses low phenacetin O-deethylation activity. Reference: "Purification and characterization of human liver microsomal cytochrome P-450 2A6." Yun C.H., Shimada T., Guengerich F.P. Mol. Pharmacol. 40:679-685(1991). Function: nan