CD276 molecule(CD276), partial (Active), recombinant Macaca fascicularis

Organism: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey). Source: Mammalian cell. Expression Region: 29-465aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: LEVQVPEDPV VALVGTDATL RCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FTEGRDQGSA YANRTALFLD LLAQGNASLR LQRVRVADEG SFTCFVSIRD FGSAAVSLQV AAPYSKPSMT LEPNKDLRPG DTVTITCSSY RGYPEAEVFW QDGQGAPLTG NVTTSQMANE QGLFDVHSVL RVVLGANGTY SCLVRNPVLQ QDAHGSITIT PQRSPTGAVE VQVPEDPVVA LVGTDATLRC SFSPEPGFSL AQLNLIWQLT DTKQLVHSFT EGRDQGSAYA NRTALFLDLL AQGNASLRLQ RVRVADEGSF TCFVSIRDFG SAAVSLQVAA PYSKPSMTLE PNKDLRPGDT VTITCSSYRG YPEAEVFWQD GQGAPLTGNV TTSQMANEQG LFDVHSVLRV VLGANGTYSC LVRNPVLQQD AHGSVTITGQ PMTFPPE. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD276 at 2 µg/mL can bind Anti-CD276 recombinant antibody(CSB-RA733578MA1HU). The EC50 is 4.299-5.373 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: n/a. Reference: Warren W., Wilson R.K. Submitted to EMBL/GenBank/DDBJ databases (MAR-2013). Function: nan