Catalase (CAT), partial, dog, recombinant

Organism: Canis lupus familiaris (Dog) (Canis familiaris). Source: E.coli. Expression Region: 35-527aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: IGDKLNVMTA GPRGPLLVQD VVFTDEMAHF DRERIPERVV HAKGAGAFGY FEVTHDITKY SKAKVFEHIG KRTPIAVRFS TVAGESGSAD TVRDPRGFAV KFYTEDGNWD LVGNNTPIFF IRDAILFPSF IHSQKRNPQT HLKDPDMVWD FWSLRPESLH QVSFLFSDRG IPDGHRHMNG YGSHTFKLVN AAGEAVYCKF HYKTDQGIKN LSVEDAARLS HEDPDYGLRD LFNAIATGNY PSWTFYIQVM TFSQAETFPF NPFDLTKIWP HQDYPLIPVG KLVLNRNPVN YFAEVEQMAF DPSNMPPGIE PSPDKMLQGR LFAYPDTHRH RLGPNYLQIP VNCPFRARVA NYQRDGPMCM LDNQGGAPNY YPNSFSAPEQ QRCVLEHSSQ CSPDVQRFNS ANEDNVTQVR TFYLKVLGEE ERKRLCENIA GHLKDAQLFI QKKAVKNFSD VHPDYGARIQ ALLDKYNAEK PKNAIHTFMQ HGSHLAAREK ANL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells. Reference: "cDNA cloning of mutant catalase in acatalasemic beagle dog: single nucleotide substitution leading to thermal-instability and enhanced proteolysis of mutant enzyme." Nakamura K., Watanabe M., Takanaka K., Sasaki Y., Ikeda T. Int. J. Biochem. Cell Biol. 32:1183-1193(2000). Function: nan