Catalase (Cat), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 2-527aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal myc-tagged. Target Protein Sequence: SDSRDPASDQ MKQWKEQRAS QRPDVLTTGG GNPIGDKLNI MTAGSRGPLL VQDVVFTDEM AHFDRERIPE RVVHAKGAGA FGYFEVTHDI TRYSKAKVFE HIGKRTPIAV RFSTVTGESG SADTVRDPRG FAVKFYTEDG NWDLVGNNTP IFFIRDAILF PSFIHSQKRN PQTHLKDPDM VWDFWSLRPE SLHQVSFLFS DRGIPDGHRH MNGYGSHTFK LVNADGEAVY CKFHYKTDQG IKNLPVGEAG RLAQEDPDYG LRDLFNAIAN GNYPSWTFYI QVMTFKEAET FPFNPFDLTK VWPHKDYPLI PVGKLVLNKN PVNYFAEVEQ MAFDPSNMPP GIEPSPDKML QGRLFAYPDT HRHRLGPNYL QIPVNCPYRA RVANYQRDGP MCMHDNQGGA PNYYPNSFSA PEQQRSALEH SVQCAVDVKR FNSANEDNVT QVRTFYTKVL NEEERKRLCE NIAGHLKDAQ LFIQKKAVKN FTDVHPDYGA RIQALLDKYN AEKPKNAIHT YTQAGSHMAA KGKANL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells. Reference: "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009). Function: nan