Carbonic anhydrase-related protein (CA8), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-290aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: ADLSFIEDTV AFPEKEEDEE EEEEGVEWGY EEGVEWGLVF PDANGEYQSP INLNSREARY DPSLLDVRLS PNYVVCRDCE VTNDGHTIQV ILKSKSVLSG GPLPQGHEFE LYEVRFHWGR ENQRGSEHTV NFKAFPMELH LIHWNSTLFG SIDEAVGKPH GIAIIALFVQ IGKEHVGLKA VTEILQDIQY KGKSKTIPCF NPNTLLPDPL LRDYWVYEGS LTIPPCSEGV TWILFRYPLT ISQLQIEEFR RLRTHVKGAE LVEGCDGILG DNFRPTQPLS DRVIRAAFQ. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/µg as determined by LAL method. Biological Activity: The esterase activity is determined to be is 162.5 pmol/min/µg. Form: Liquid. Buffer: 0.2 µm filtered 20 mM Tris-HCl, 500 mM NaCl, 1 mM DTT, pH 8.5. Reconstitution: n/a. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Carbonic Anhydrase 8 (CA8) belongs to the alpha-carbonic anhydrase family. Alpha-carbonic anhydrase is a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. Because CA8 has some sequence similarity with other known carbonic anhydrase genes, it was firstly called as CA-related protein. Nevertheless, CA8 does not have a carbonic anhydrase catalytic activity. Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3), which is a congenital cerebellar ataxia associated with dysarthia, quadrupedal gait and mild mental retardation. Reference: n/a. Function: Does not have a carbonic anhydrase catalytic activity.