Carbonic anhydrase 2 (Ca2), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Yeast. Expression Region: 2-260aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: SHHWGYSKSN GPENWHKEFP IANGDRQSPV DIDTGTAQHD PSLQPLLICY DKVASKSIVN NGHSFNVEFD DSQDFAVLKE GPLSGSYRLI QFHFHWGSSD GQGSEHTVNK KKYAAELHLV HWNTKYGDFG KAVQHPDGLA VLGIFLKIGP ASQGLQKITE ALHSIKTKGK RAAFANFDPC SLLPGNLDYW TYPGSLTTPP LLECVTWIVL KEPITVSSEQ MSHFRKLNFN SEGEAEELMV DNWRPAQPLK NRKIKASFK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. Reference: "Carbonic anhydrase II plays a major role in osteoclast differentiation and bone resorption by effecting the steady state intracellular pH and Ca2+." Lehenkari P., Hentunen T.A., Laitala-Leinonen T., Tuukkanen J., Vaeaenaenen H.K. Exp. Cell Res. 242:128-137(1998). Function: nan