Cadherin-6(Cdh6), partial (Active), recombinant rat

Organism: Rattus norvegicus (Rat). Source: Mammalian cell. Expression Region: 54-615aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: SWMWNQFFLL EEYTGSDYQY VGKLHSDQDR GDGSLKYILS GDGAGDLFII NENTGDIQAT KRLDREEKPV YILRAQAINR RTGRPVEPES EFIIKIHDIN DNEPIFTKDV YTATVPEMAD VGTFVVQVTA TDADDPTYGN SAKVVYSILQ GQPYFSVESE TGIIKTALLN MDRENREQYQ VVIQAKDMGG QMGGLSGTTT VNITLTDVND NPPRFPQSTY QFKTPESSPP GTPIGRIKAS DADVGENAEI EYSITDGEGH DMFDVITDQE TQEGIITVKK LLDFEKKRVY TLKVEASNPH IEPRFLYLGP FKDSATVRIV VDDVDEPPVF SKPAYILQIR EDAQINTTIG SVAAQDPDAA RNPVKYSVDR HTDMDRIFNI DSGNGSIFTS KLLDRETLLW HNITVIATEI NNPKQSSRVP LYIKVLDVND NAPEFAEFYE TFVCEKAKAD QLIQTLHAVD KDDPYSGHQF SFSLAPEAAS GSNFTIQDNK DNTAGILTRK NGYNRHEMST YLLPVVISDN DYPVQSSTGT VTVRVCACDH HGNMQSCHAE ALIHPTGLST GA. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Rat CDH6 at 2 µg/mL can bind Anti-CDH6 recombinant antibody (CSB-RA005055MA1HU). The EC50 is 1.630-1.837 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Reference: Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V. Nat. Commun. 3:876-876 (2012). Function: nan