Atrial natriuretic peptide receptor 1 (NPR1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 33-473aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: GNLTVAVVLP LANTSYPWSW ARVGPAVELA LAQVKARPDL LPGWTVRTVL GSSENALGVC SDTAAPLAAV DLKWEHNPAV FLGPGCVYAA APVGRFTAHW RVPLLTAGAP ALGFGVKDEY ALTTRAGPSY AKLGDFVAAL HRRLGWERQA LMLYAYRPGD EEHCFFLVEG LFMRVRDRLN ITVDHLEFAE DDLSHYTRLL RTMPRKGRVI YICSSPDAFR TLMLLALEAG LCGEDYVFFH LDIFGQSLQG GQGPAPRRPW ERGDGQDVSA RQAFQAAKII TYKDPDNPEY LEFLKQLKHL AYEQFNFTME DGLVNTIPAS FHDGLLLYIQ AVTETLAHGG TVTDGENITQ RMWNRSFQGV TGYLKIDSSG DRETDFSLWD MDPENGAFRV VLNYNGTSQE LVAVSGRKLN WPLGYPPPDI PKCGFDNEDP ACNQDHLSTL E. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand. Reference: "Expression of mRNA for atrial natriuretic peptide receptor guanylate cyclase (ANPRA) in human retina." Pardhasaradhi K., Kutty R.K., Gentleman S., Krishna G. Cell. Mol. Neurobiol. 14:1-7(1994). Function: nan