Apolipoprotein A-IV (Apoa4), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 21-395aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: EVTSDQVANV VWDYFTQLSN NAKEAVEQFQ KTDVTQQLST LFQDKLGDAS TYADGVHNKL VPFVVQLSGH LAQETERVKE EIKKELEDLR DRMMPHANKV TQTFGENMQK LQEHLKPYAV DLQDQINTQT QEMKLQLTPY IQRMQTTIKE NVDNLHTSMM PLATNLKDKF NRNMEELKGH LTPRANELKA TIDQNLEDLR RSLAPLTVGV QEKLNHQMEG LAFQMKKNAE ELQTKVSAKI DQLQKNLAPL VEDVQSKVKG NTEGLQKSLE DLNRQLEQQV EEFRRTVEPM GEMFNKALVQ QLEQFRQQLG PNSGEVESHL SFLEKSLREK VNSFMSTLEK KGSPDQPQAL PLPEQAQEQA QEQAQEQVQP KPLES. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II, potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Reference: Mass spectral analysis of the apolipoproteins on mouse high density lipoproteins. Detection of post-translational modifications.Puppione D.L., Yam L.M., Bassilian S., Souda P., Castellani L.W., Schumaker V.N., Whitelegge J.P.Biochim. Biophys. Acta 1764:1363-1371(2006). Function: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II, potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.