Alkaline phosphatase, placental type (ALPP),partial, human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 117-447aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: TATAYLCGVK GNFQTIGLSA AARFNQCNTT RGNEVISVMN RAKKAGKSVG VVTTTRVQHA SPAGTYAHTV NRNWYSDADV PASARQEGCQ DIATQLISNM DIDVILGGGR KYMFRMGTPD PEYPDDYSQG GTRLDGKNLV QEWLAKRQGA RYVWNRTELM QASLDPSVTH LMGLFEPGDM KYEIHRDSTL DPSLMEMTEA ALRLLSRNPR GFFLFVEGGR IDHGHHESRA YRALTETIMF DDAIERAGQL TSEEDTLSLV TADHSHVFSF GGYPLRGSSI FGLAPGKARD RKAYTVLLYG NGPGYVLKDG ARPDVTESES GSPEYRQQSA V. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Reference: "Nucleotide sequence of the human placental alkaline phosphatase gene. Evolution of the 5' flanking region by deletion/substitution." Knoll B.J., Rothblum K.N., Longley M.A. J. Biol. Chem. 263:12020-12027(1988). Function: nan