Alkaline phosphatase, germ cell type (ALPG) (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 20-503aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: IIPVEEENPD FWNRQAAEAL GAAKKLQPAQ TAAKNLIIFL GDGMGVSTVT AARILKGQKK DKLGPETFLA MDRFPYVALS KTYSVDKHVP DSGATATAYL CGVKGNFQTI GLSAAARFNQ CNTTRGNEVI SVMNRAKKAG KSVGVVTTTR VQHASPAGAY AHTVNRNWYS DADVPASARQ EGCQDIATQL ISNMDIDVIL GGGRKYMFPM GTPDPEYPDD YSQGGTRLDG KNLVQEWLAK HQGARYVWNR TELLQASLDP SVTHLMGLFE PGDMKYEIHR DSTLDPSLME MTEAALLLLS RNPRGFFLFV EGGRIDHGHH ESRAYRALTE TIMFDDAIER AGQLTSEEDT LSLVTADHSH VFSFGGYPLR GSSIFGLAPG KARDRKAYTV LLYGNGPGYV LKDGARPDVT ESESGSPEYR QQSAVPLDGE THAGEDVAVF ARGPQAHLVH GVQEQTFIAH VMAFAACLEP YTACDLAPRA GTTD. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ALPG at 2 µg/mL can bind Anti-ALPG recombinant antibody (CSB-RA001633MA1HU), the EC50 is 14.09-23.17 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May hydrolyze various phosphate compounds. Reference: "Mutation of a single amino acid converts germ cell alkaline phosphatase to placental alkaline phosphatase." Watanabe T., Wada N., Kim E.E., Wyckoff H.W., Chou J.Y. J. Biol. Chem. 266:21174-21178(1991). Function: nan