Aldehyde dehydrogenase, mitochondrial (Aldh2), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 20-519aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SAAATSAVPA PNQQPEVFCN QIFINNEWHD AVSKKTFPTV NPSTGEVICQ VAEGNKEDVD KAVKAAQAAF QLGSPWRRMD ASDRGRLLYR LADLIERDRT YLAALETLDN GKPYVISYLV DLDMVLKCLR YYAGWADKYH GKTIPIDGDF FSYTRHEPVG VCGQIIPWNF PLLMQAWKLG PALATGNVVV MKVAEQTPLT ALYVANLIKE AGFPPGVVNI VPGFGPTAGA AIASHEDVDK VAFTGSTEVG HLIQVAAGSS NLKRVTLELG GKSPNIIMSD ADMDWAVEQA HFALFFNQGQ CCCAGSRTFV QEDVYDEFVE RSVARAKSRV VGNPFDSRTE QGPQVDETQF KKILGYIKSG QQEGAKLLCG GGAAADRGYF IQPTVFGDVK DGMTIAKEEI FGPVMQILKF KTIEEVVGRA NNSKYGLAAA VFTKDLDKAN YLSQALQAGT VWINCYDVFG AQSPFGGYKM SGSGRELGEY GLQAYTEVKT VTVKVPQKNS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: "Mutations in mitochondrial aldehyde dehydrogenase (ALDH2) change cofactor affinity and segregate with voluntary alcohol consumption in rats." Sapag A., Tampier L., Valle-Prieto A., Quintanilla M.E., Moncada C., Israel Y. Pharmacogenetics 13:509-515(2003). Function: nan