Alanine aminotransferase 1 (GPT), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-496aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: ASSTGDRSQA VRHGLRAKVL TLDGMNPRVR RVEYAVRGPI VQRALELEQE LRQGVKKPFT EVIRANIGDA QAMGQRPITF LRQVLALCVN PDLLSSPNFP DDAKKRAERI LQACGGHSLG AYSVSSGIQL IREDVARYIE RRDGGIPADP NNVFLSTGAS DAIVTVLKLL VAGEGHTRTG VLIPIPQYPL YSATLAELGA VQVDYYLDEE RAWALDVAEL HRALGQARDH CRPRALCVIN PGNPTGQVQT RECIEAVIRF AFEERLFLLA DEVYQDNVYA AGSQFHSFKK VLMEMGPPYA GQQELASFHS TSKGYMGECG FRGGYVEVVN MDAAVQQQML KLMSVRLCPP VPGQALLDLV VSPPAPTDPS FAQFQAEKQA VLAELAAKAK LTEQVFNEAP GISCNPVQGA MYSFPRVQLP PRAVERAQEL GLAPDMFFCL RLLEETGICV VPGSGFGQRE GTYHFRMTIL PPLEKLRLLL EKLSRFHAKF TLEYS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles . Reference: "Joubert Syndrome in French Canadians and Identification of Mutations in CEP104." Care4Rare Canada Consortium Srour M., Hamdan F.F., McKnight D., Davis E., Mandel H., Schwartzentruber J., Martin B., Patry L., Nassif C., Dionne-Laporte A., Ospina L.H., Lemyre E., Massicotte C., Laframboise R., Maranda B., Labuda D., Decarie J.C., Rypens F. Michaud J.L. Am. J. Hum. Genet. 97:744-753(2015). Function: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).