Acetylcholinesterase (Ache), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 32-614aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: EGREDPQLLV RVRGGQLRGI RLKAPGGPVS AFLGIPFAEP PVGSRRFMPP EPKRPWSGIL DATTFQNVCY QYVDTLYPGF EGTEMWNPNR ELSEDCLYLN VWTPYPRPTS PTPVLIWIYG GGFYSGASSL DVYDGRFLAQ VEGTVLVSMN YRVGTFGFLA LPGSREAPGN VGLLDQRLAL QWVQENIAAF GGDPMSVTLF GESAGAASVG MHILSLPSRS LFHRAVLQSG TPNGPWATVS AGEARRRATL LARLVGCPPG GAGGNDTELI SCLRTRPAQD LVDHEWHVLP QESIFRFSFV PVVDGDFLSD TPDALINTGD FQDLQVLVGV VKDEGSYFLV YGVPGFSKDN ESLISRAQFL AGVRIGVPQA SDLAAEAVVL HYTDWLHPED PAHLRDAMSA VVGDHNVVCP VAQLAGRLAA QGARVYAYIF EHRASTLTWP LWMGVPHGYE IEFIFGLPLD PSLNYTVEER IFAQRLMQYW TNFARTGDPN DPRDSKSPRW PPYTTAAQQY VSLNLKPLEV RRGLRAQTCA FWNRFLPKLL SATDTLDEAE RQWKAEFHRW SSYMVHWKNQ FDHYSKQERC SDL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Reference: "Effect of acute noise stress on acetylcholinesterase activity in discrete areas of rat brain." Sembulingam K., Sembulingam P., Namasivayam A. Indian J Med Sci 57:487-492(2003). Function: nan