Acetylcholinesterase (ACHE), bovine, recombinant

Organism: Bos taurus (Bovine). Source: E.coli. Expression Region: 31-613aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal GST-tagged. Target Protein Sequence: EGPEDPELLV MVRGGRLRGL RLMAPRGPVS AFLGIPFAEP PVGPRRFLPP EPKRPWPGVL NATAFQSVCY QYVDTLYPGF EGTEMWNPNR ELSEDCLYLN VWTPYPRPSS PTPVLVWIYG GGFYSGASSL DVYDGRFLTQ AEGTVLVSMN YRVGAFGFLA LPGSREAPGN VGLLDQRLAL QWVQENVAAF GGDPTSVTLF GESAGAASVG MHLLSPPSRG LFHRAVLQSG APNGPWATVG VGEARRRATL LARLVGCPPG GAGGNDTELV ACLRARPAQD LVDHEWRVLP QESVFRFSFV PVVDGDFLSD TPEALINAGD FHGLQVLVGV VKDEGSYFLV YGAPGFSKDN ESLISRAQFL AGVRVGVPQA SDLAAEAVVL HYTDWLHPED PARLREALSD VVGDHNVVCP VAQLAGRLAA QGARVYAYIF EHRASTLSWP LWMGVPHGYE IEFIFGLPLE PSLNYTIEER TFAQRLMRYW ANFARTGDPN DPRDPKAPQW PPYTAGAQQY VSLNLRPLEV RRGLRAQACA FWNRFLPKLL SATDTLDEAE RQWKAEFHRW SSYMVHWKNQ FDHYSKQDRC SDL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Reference: Bovine acetylcholinesterase cloning, expression and characterization.Mendelson I., Kronman C., Ariel N., Shafferman A., Velan B.Biochem. J. 334:251-259(1998). Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.