Acetyl-CoA carboxylase (ACC1), partial, Saccharomyces cerevisiae, recombinant

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). Source: E.coli. Expression Region: 217-775aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: TGLVSVDDDI YQKGCCTSPE DGLQKAKRIG FPVMIKASEG GGGKGIRQVE REEDFIALYH QAANEIPGSP IFIMKLAGRA RHLEVQLLAD QYGTNISLFG RDCSVQRRHQ KIIEEAPVTI AKAETFHEME KAAVRLGKLV GYVSAGTVEY LYSHDDGKFY FLELNPRLQV EHPTTEMVSG VNLPAAQLQI AMGIPMHRIS DIRTLYGMNP HSASEIDFEF KTQDATKKQR RPIPKGHCTA CRITSEDPND GFKPSGGTLH ELNFRSSSNV WGYFSVGNNG NIHSFSDSQF GHIFAFGENR QASRKHMVVA LKELSIRGDF RTTVEYLIKL LETEDFEDNT ITTGWLDDLI THKMTAEKPD PTLAVICGAA TKAFLASEEA RHKYIESLQK GQVLSKDLLQ TMFPVDFIHE GKRYKFTVAK SGNDRYTLFI NGSKCDIILR QLSDGGLLIA IGGKSHTIYW KEEVAATRLS VDSMTTLLEV ENDPTQLRTP SPGKLVKFLV ENGEHIIKGQ PYAEIEVMKM QMPLVSQENG IVQLLKQPGS TIVAGDIMAI MTLDDPSKV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Involved in the synthesis of very-long-chain fatty acid synthesis which is required to maintain a functional nuclear envelope. Required for acylation and vacuolar membrane association of VAC8 which is necessary to maintain a normal morphology of the vacuole. Reference: "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997). Function: Carries out three functions