Acetyl-CoA carboxylase 1 (Acaca), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 116-617aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: VIEKVLIANN GIAAVKCMRS IRRWSYEMFR NERAIRFVVM VTPEDLKANA EYIKMADHYV PVPGGANNNN YANVELILDI AKRIPVQAVW AGWGHASENP KLPELLLKNG IAFMGPPSQA MWALGDKIAS SIVAQTAGIP TLPWSGSGLR VDWQENDFSK RILNVPQDLY EKGYVKDVDD GLKAAEEVGY PVMIKASEGG GGKGIRKVNN ADDFPNLFRQ VQAEVPGSPI FVMRLAKQSR HLEVQILADQ YGNAISLFGR DCSVQRRHQK IIEEAPAAIA TPAVFEHMEQ CAVKLAKMVG YVSAGTVEYL YSQDGSFYFL ELNPRLQVEH PCTEMVADVN LPAAQLQIAM GIPLFRIKDI RMMYGVSPWG DAPIDFENSA HVPCPRGHVI AARITSENPD EGFKPSSGTV QELNFRSNKN VWGYFSVAAA GGLHEFADSQ FGHCFSWGEN REEAISNMVV ALKELSIRGD FRTTVEYLIK LLETESFQLN RIDTGWLDRL IA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Reference: "Structure of the coding sequence and primary amino acid sequence of acetyl-coenzyme A carboxylase."Lopez-Casillas F., Bai D.-H., Luo X., Kong I.-S., Hermodson M.A., Kim K.-H.Proc. Natl. Acad. Sci. U.S.A. 85:5784-5788(1988). Function: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions