Acetyl-CoA carboxylase 1 (ACACA), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 78-617aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: SMSGLHLVKQ GRDRKKIDSQ RDFTVASPAE FVTRFGGNKV IEKVLIANNG IAAVKCMRSI RRWSYEMFRN ERAIRFVVMV TPEDLKANAE YIKMADHYVP VPGGPNNNNY ANVELILDIA KRIPVQAVWA GWGHASENPK LPELLLKNGI AFMGPPSQAM WALGDKIASS IVAQTAGIPT LPWSGSGLRV DWQENDFSKR ILNVPQELYE KGYVKDVDDG LQAAEEVGYP VMIKASEGGG GKGIRKVNNA DDFPNLFRQV QAEVPGSPIF VMRLAKQSRH LEVQILADQY GNAISLFGRD CSVQRRHQKI IEEAPATIAT PAVFEHMEQC AVKLAKMVGY VSAGTVEYLY SQDGSFYFLE LNPRLQVEHP CTEMVADVNL PAAQLQIAMG IPLYRIKDIR MMYGVSPWGD SPIDFEDSAH VPCPRGHVIA ARITSENPDE GFKPSSGTVQ ELNFRSNKNV WGYFSVAAAG GLHEFADSQF GHCFSWGENR EEAISNMVVA LKELSIRGDF RTTVEYLIKL LETESFQMNR IDTGWLDRLI. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. Reference: "Fine expression profiling of full-length transcripts using a size-unbiased cDNA library prepared with the vector-capping method." Oshikawa M., Sugai Y., Usami R., Ohtoko K., Toyama S., Kato S. DNA Res. 15:123-136(2008). Function: nan