ACC-1, active human recombinant protein

Human ACC1 (acetyl-Coenzyme A carboxylase alpha transcript variant 1) (GenBank Accession No. NM_198834), amino acids 1-2383(end) with C-terminal FLAG and His-tags, MW = 270 kDa, expressed in Baculovirus infected Sf9 cell expression system. A 25 µl ACC1 reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 µM ATP, 12mM NaHCO3 and 20 µM acetyl-CoA at 30oC for 45 min. ATP reduction is detected using ADP-Glo Luminescent Assay Platform (Promega).