5-Lipoxygenase (human, recombinant)

5-Lipoxygenase (5-LO) is an enzyme encoded by ALOX5 in humans that is involved in leukotriene biosynthesis. It is composed of an N-terminal regulatory domain and a C-terminal catalytic domain and is primarily expressed in leukocytes. Increases in intracellular calcium levels or cellular stress induce translocation of 5-LO from the cytosol or nucleoplasm, depending on the cell type and 5-LO phosphorylation status, to the nuclear envelope, where it interacts with 5-LO-activating protein (FLAP), which transfers arachidonic acid (Cay-90010, Cay-90010.1, Cay-10006607) to 5-LO. 5-LO catalyzes the conversion of arachidonic acid to 5(S)-HpETE (Cay-44230) and then to leukotriene A4 (LTA4). Other substrates of 5-LO include 5,8,11,14,17-eicosapentaenoic acid, 5,8,11-eicosatrienoic acid, 5,8-eicosadienoic acid, 12-HpETE, and 15-HpETE. Alox5 knockout mice are protected against arthritis and pulmonary inflammation. Knockout of Alox5 also protects apolipoprotein E-deficient hyperlipidemic mice from high-fat diet-induced hepatic injury and inflammation. Levels of 5-LO are elevated in postmortem hippocampus and cortex of patients with Alzheimer's disease. Cayman's 5-Lipoxygenase (human, recombinant) can be used for enzyme activity assays.Synonyms: 5-LO, 5-LOX, ALOX5, Arachidonate 5-lipoxygenase, LOG5. Purity: Clarified lysate. Source: Active recombinant human 5-lipoxygenase expressed in insect cells. Amino Acids: 1-674 (full length). MW: 78 kDa. Formulation: (Request formulation change), 100 mM Tris-HCl, pH 8.0, with 5 mM EGTA, 1mM calcium chloride, and 30% glycerol. Host: Insect cells. Enzyme Commission: 1.13.11.34. UniProt Accession #: P09917.