5-hydroxytryptamine receptor 2C (Htr2c), recombinant mouse 5-hydroxytryptamine receptor

Organism: Mus musculus (Mouse). Source: in vitro E.coli expression system. Expression Region: 33-459aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: IVTDTFNSSD GGRLFQFPDG VQNWPALSIV VIIIMTIGGN ILVIMAVSME KKLHNATNYF LMSLAIADML VGLLVMPLSL LAILYDYVWP LPRYLCPVWI SLDVLFSTAS IMHLCAISLD RYVAIRNPIE HSRFNSRTKA IMKIAIVWAI SIGVSVPIPV IGLRDESKVF VNNTTCVLND PNFVLIGSFV AFFIPLTIMV ITYFLTIYVL RRQTLMLLRG HTEEELRNIS LNFLKCCCKK GDEEENAPNP NPDQKPRRKK KEKRPRGTMQ AINNEKKASK VLGIVFFVFL IMWCPFFITN ILSVLCGKAC NQKLMEKLLN VFVWIGYVCS GINPLVYTLF NKIYRRAFSK YLRCDYKPDK KPPVRQIPRV AATALSGREL NVNIYRHTNE RVVRKANDTE PGIEMQVENL ELPVNPSNVV SERISSV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: G-protein coupled receptor for 5-hydroxytryptamine serotonin. Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane DOI and lysergic acid diethylamide LSD. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins G proteins and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and feeding behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis. Reference: n/a. Function: nan