1-Phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (Plcd1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-756aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MDSGRDFLTL HGLQDDPDLQ ALLKGSQLLK VKSSSWRRER FYKLQEDCKT IWQESRKVMR SPESQLFSIE DIQEVRMGHR TEGLEKFARD IPEDRCFSIV FKDQRNTLDL IAPSPADVQH WVQGLRKIID RSGSMDQRQK LQHWIHSCLR KADKNKDNKM NFKEVKDFLK ELNVQVDDSY ARKIFRECDH SQTDSLEDEE IETFYRMLTQ RAEIDRAFAE AAGSAETLSV EKLVTFLQHQ QREEEAGPAL ALSLIERYEP SETAKAQRQM TKDGFLMYLL SADGNAFSLA HRRVYQDMNQ PLSHYLVSSS HNTYLLEDQL TGPSSTEAYI RALCKGCRCL ELDCWDGPNQ EPIIYHGYTF TSKILFCDVL RAIRDYAFKA SPYPVILSLE NHCSLEQQRV MAHHLRAILG PMLLDQPLDG VTTSLPSPEQ LKEKILLKGK KLGGLLPAGG ENGPEATDVS DEDEAAEMED EAVRSQVQHK PKEDKLKLVP ELSDMVIYCK SVHFGGFSSP STSGQAFYEM ASFSESRALR LLQESGNSFV RHNVGHLSRI YPAGWRTDSS NYSPVEMWNG GCQIVALNFQ TPGPEMDVYL GCFQDNGGCG YVLKPAFLRD PDTTFNSRAL TQGPWWAPKK LRVWIISGQQ LPKVNKNKNS IVDPKVIVEI HGVGQDVASR QTAVITNNGF NPRWDTEFEF VVAVPDLALV RFMVEDYDSS SKNDFIGQST IPWNSLKQGY RHVHLLSKNG DLHPSATLFV KISIQD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development. Binds phosphatidylinositol 4,5-bisphosphate. Reference: "Phospholipase C-delta1 and -delta3 are essential in the trophoblast for placental development." Nakamura Y., Hamada Y., Fujiwara T., Enomoto H., Hiroe T., Tanaka S., Nose M., Nakahara M., Yoshida N., Takenawa T., Fukami K. Mol. Cell. Biol. 25:10979-10988(2005). Function: nan