Z-LRGG-AMC (trifluoroacetate salt)

Z-LRGG-AMC is a fluorogenic substrate for isopeptidase T. Upon enzymatic cleavage by isopeptidase T, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify isopeptidase T activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.Formal Name: N-[(phenylmethoxy)carbonyl]-L-leucyl-L-arginylglycyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-glycinamide, trifluoroacetate salt. Synonyms: Z-Leu-Arg-Gly-Gly-AMC, Z-Leu-Arg-Gly-Gly-7-amino-4-methylcoumarin. Molecular Formula: C34H44N8O8 . XCF3COOH. Formula Weight: 692.8. Purity: >95%. Emission: 440-460 nm. Excitation: 340-360 nm. Formulation: (Request formulation change), A solid. Solubility: DMF: 20 mg/ml, DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml, DMSO: 16 mg/ml. lambdamax: 327 nm. SMILES: O=C1C=C(C)C2=C(C=C(NC(CNC(CNC([C@H](CCCNC(N)=N)NC([C@@H](NC(OCC3=CC=CC=C3)=O)CC(C)C)=O)=O)=O)=O)C=C2)O1.FC(F)(C(O)=O)F. InChi Code: InChI=1S/C34H44N8O8.C2HF3O2/c1-20(2)14-26(42-34(48)49-19-22-8-5-4-6-9-22)32(47)41-25(10-7-13-37-33(35)36)31(46)39-17-28(43)38-18-29(44)40-23-11-12-24-21(3)15-30(45)50-27(24)16-23,3-2(4,5)1(6)7/h4-6,8-9,11-12,15-16,20,25-26H,7,10,13-14,17-19H2,1-3H3,(H,38,43)(H,39,46)(H,40,44)(H,41,47)(H,42,48)(H4,35,36,37),(H,6,7)/t25-,26-,/m0./s1. InChi Key: OHFBLWHOBJRDAB-CCQIZPNASA-N.