Calpain Inhibitor I

Calpain inhibitor I is a synthetic tripeptide aldehyde that acts as a potent inhibitor of cysteine proteases including calpain I (Ki = 190 nM), calpain II (Ki = 220 nM), cathepsin B (Ki = 150 nM), and cathepsin L (Ki = 500 pM). At 10-100 µM calpain inhibitor I dose-dependently prevents the degradation of IkappaBalpha and IkappaBbeta by the ubiquitin-proteasome complex, which blocks activation of NFkappaB and the production of TNF and IL-1beta, suggesting a potential therapeutic effect for inflammatory diseases. At 10 µM, calpain inhibitor I can inhibit nitric oxide production by activated macrophages by interfering with transcription of the inducible nitric oxide synthase gene.Formal Name: N-acetyl-L-leucyl-N-[(1S)-1-formylpentyl]-L-leucinamide. CAS Number: 110044-82-1. Synonyms: Ac-Leu-Leu-Nle-Aldehyde, ALLN, MG 101. Molecular Formula: C20H37N3O4. Formula Weight: 383.5. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 20 mg/ml, DMSO: 20 mg/ml, Ethanol: 20 mg/ml, Ethanol:PBS (pH 7.2) (1:1): 0.5 mg/ml. SMILES: CCCC[C@H](NC([C@@H](NC([C@@H](NC(C)=O)CC(C)C)=O)CC(C)C)=O)C=O. InChi Code: InChI=1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1. InChi Key: FMYKJLXRRQTBOR-BZSNNMDCSA-N.