Bz-Nle-KRR-AMC (hydrochloride)

Bz-Nle-KRR-AMC is a fluorogenic peptide substrate for yellow fever virus (YFV) non-structural 3 (NS3) and dengue virus (DV) NS2B/3 serine proteases. Upon enzymatic cleavage by YFV NS3 or DV NS2B/3 serine proteases, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify YFV NS3 or DV NS2B/3 serine protease activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.Formal Name: N-benzoyl-L-norleucyl-L-lysyl-L-arginyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-argininamide, hydrochloride. CAS Number: 863975-32-0. Synonyms: Benzoyl-Nle-Lys-Arg-Arg-AMC, Benzoyl-Nle-Lys-Arg-Arg-7-amino-4-methylcoumarin, Bz-Nle-Lys-Arg-Arg-AMC. Molecular Formula: C41H60N12O7 . XHCl. Formula Weight: 833.0. Purity: >95%. Emission: 440-460 nm. Excitation: 340-360 nm. Formulation: (Request formulation change), A solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml, Ethanol: 20 mg/ml. lambdamax: 230, 299, 326 nm. SMILES: O=C(N[C@@H](CCCC)C(N[C@@H](CCCCN)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCNC(N)=N)C(NC1=CC=C(C(C)=CC(O2)=O)C2=C1)=O)=O)=O)=O)C3=CC=CC=C3.Cl. InChi Code: InChI=1S/C41H60N12O7.ClH/c1-3-4-14-29(50-35(55)26-12-6-5-7-13-26)37(57)51-30(15-8-9-20-42)38(58)53-32(17-11-22-48-41(45)46)39(59)52-31(16-10-21-47-40(43)44)36(56)49-27-18-19-28-25(2)23-34(54)60-33(28)24-27,/h5-7,12-13,18-19,23-24,29-32H,3-4,8-11,14-17,20-22,42H2,1-2H3,(H,49,56)(H,50,55)(H,51,57)(H,52,59)(H,53,58)(H4,43,44,47)(H4,45,46,48),1H/t29-,30-,31-,32-,/m0./s1. InChi Key: DINNPVBPBDJKQJ-WYDLTDSDSA-N.