Ac-WEHD-AFC (trifluoroacetate salt)

Ac-WEHD-AFC is a fluorogenic substrate that can be cleaved by group I caspases (caspase-1, -4, and -5). Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.Formal Name: N-acetyl-L-tryptophyl-L-alpha-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-alpha-asparagine, trifluoroacetate salt. Synonyms: N-Acetyl-Trp-Glu-His-Asp-7-amino-4-Trifluoromethylcoumarin, Caspase-1 Substrate (Fluorogenic), Caspase-5 Substrate (Fluorogenic). Molecular Formula: C38H37F3N8O11 . XCF3COOH. Formula Weight: 838.7. Purity: >95%. Emission: 505 nm. Excitation: 400 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 10 mg/ml, Water: 1 mg/ml. lambdamax: 210, 338 nm. SMILES: OC(C[C@H](NC([C@@H](NC([C@@H](NC([C@H](CC1=CNC2=C1C=CC=C2)NC(C)=O)=O)CCC(O)=O)=O)CC3=CNC=N3)=O)C(NC(C=C4O5)=CC=C4C(C(F)(F)F)=CC5=O)=O)=O.FC(F)(C(O)=O)F. InChi Code: InChI=1S/C38H37F3N8O11.C2HF3O2/c1-18(50)45-27(10-19-15-43-25-5-3-2-4-22(19)25)36(58)47-26(8-9-31(51)52)34(56)48-28(11-21-16-42-17-44-21)37(59)49-29(14-32(53)54)35(57)46-20-6-7-23-24(38(39,40)41)13-33(55)60-30(23)12-20,3-2(4,5)1(6)7/h2-7,12-13,15-17,26-29,43H,8-11,14H2,1H3,(H,42,44)(H,45,50)(H,46,57)(H,47,58)(H,48,56)(H,49,59)(H,51,52)(H,53,54),(H,6,7)/t26-,27-,28-,29-,/m0./s1. InChi Key: UELPUHYAUOLMOR-MROKUPSKSA-N.