Ac-VDVAD-AFC (trifluoroacetate salt)

Ac-VDVAD-AFC is a fluorogenic substrate for caspase-2. Upon enzymatic cleavage by caspase-2, 7-amino-4-trifluoromethylcoumarin (AFC) is released and its fluorescence can be used to quantify caspase-2 activity. AFC displays excitation/emission maxima of 400/505 nm, respectively.Formal Name: N-acetyl-L-valyl-L-alpha-aspartyl-L-valyl-L-alanyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-alpha-asparagine, trifluoroacetate salt. Synonyms: N-Acetyl-Val-Asp-Val-Ala-Asp-AFC, N-Acetyl-Val-Asp-Val-Ala-Asp-7-amino-4-Trifluoromethylcoumarin, Caspase-2 Substrate (Fluorogenic). Molecular Formula: C33H41F3N6O12 . XCF3COOH. Formula Weight: 770.7. Purity: >95%. Emission: 505 nm. Excitation: 400 nm. Formulation: (Request formulation change), A solid. Solubility: Water: Soluble. SMILES: OC(C[C@H](NC([C@H](C)NC([C@@H](NC([C@H](CC(O)=O)NC([C@@H](NC(C)=O)C(C)C)=O)=O)C(C)C)=O)=O)C(NC(C=C1O2)=CC=C1C(C(F)(F)F)=CC2=O)=O)=O.OC(C(F)(F)F)=O. InChi Code: InChI=1S/C33H41F3N6O12.C2HF3O2/c1-13(2)26(38-16(6)43)32(53)41-21(12-24(46)47)30(51)42-27(14(3)4)31(52)37-15(5)28(49)40-20(11-23(44)45)29(50)39-17-7-8-18-19(33(34,35)36)10-25(48)54-22(18)9-17,3-2(4,5)1(6)7/h7-10,13-15,20-21,26-27H,11-12H2,1-6H3,(H,37,52)(H,38,43)(H,39,50)(H,40,49)(H,41,53)(H,42,51)(H,44,45)(H,46,47),(H,6,7)/t15-,20-,21-,26-,27-,/m0./s1. InChi Key: PNDAWJNREIOBPU-IQYSWUAISA-N.