Ac-Nle-Pro-Nle-Asp-AMC (trifluoroacetate salt)

Ac-Nle-Pro-Nle-Asp-AMC is a substrate for proteasomes that is cleaved by the caspase-like site, also known as postglutamyl peptide hydrolase (PGPH), with specific activities of 113 and 6.6 nmol/min/mg by the 26S (rabbit muscle) and 20S (yeast) proteasomes, respectively. Caspase-like activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm. Ac-Nle-Pro-Nle-Asp-AMC inhibits the chymotrypsin-like activity of the proteasome allosterically when bound to the caspase-like site. It has been used to determine L-DOPA's (Cay-13248) effects on protein turnover rates.Formal Name: N-acetyl-L-norleucyl-L-prolyl-L-norleucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-alpha-asparagine, trifluoroacetate salt. Synonyms: Ac-Nle-Pro-Nle-Asp-7-amido-4-Methylcoumarin, Ac-nLPnLD-AMC. Molecular Formula: C33H45N5O9 . XCF3COOH. Formula Weight: 655.7. Purity: >98%. Emission: 440-460 nm. Excitation: 340-360 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: 30 mg/ml, Ethanol:PBS (pH 7.2) (1:3): 0.25 mg/ml. lambdamax: 278, 316 nm. SMILES: O=C1C=C(C2=C(O1)C=C(C=C2)NC([C@@H](NC([C@@H](NC([C@@H]3CCCN3C([C@@H](NC(C)=O)CCCC)=O)=O)CCCC)=O)CC(O)=O)=O)C.OC(C(F)(F)F)=O. InChi Code: InChI=1S/C33H45N5O9.C2HF3O2/c1-5-7-10-23(36-32(45)26-12-9-15-38(26)33(46)24(11-8-6-2)34-20(4)39)30(43)37-25(18-28(40)41)31(44)35-21-13-14-22-19(3)16-29(42)47-27(22)17-21,3-2(4,5)1(6)7/h13-14,16-17,23-26H,5-12,15,18H2,1-4H3,(H,34,39)(H,35,44)(H,36,45)(H,37,43)(H,40,41),(H,6,7)/t23-,24-,25-,26-,/m0./s1. InChi Key: XUTUJUTTZZCAAT-KRZJFFIJSA-N.