ATG4B (human recombinant)

Autophagy-related 4B (ATG4B), also known as autophagin-1, is a cysteine protease and a member of the C54 protease family with dual roles in the progression of autophagy. It catalyzes the irreversible proteolytic cleavage of human homologs of yeast atg8 belonging to the microtubule-associated proteins 1 (MAP1)A/B-light chain 3 (LC3) and the GABA receptor-associated protein (GABARAP) subfamilies, including LC3, GATE16, GABARAP, and ATG8L, to expose a C-terminal glycine residue that is essential to formation of the autophagosome. It also catalyzes the reversible deconjugation of phosphatidylethanolamine from C-terminal glycine lipid-conjugated atg8 homologs. ATG4B expression is increased in CD34+ chronic myeloid leukemia (CML) stem and progenitor cells and knockdown of ATG4B expression increases accumulation of LC3-II and p62, indicating impaired autophagy, and reduces the viability and inhibits proliferation of CD34+ CML cells. Atg4-/- mice exhibit systemic decreases in basal and induced autophagy as well as increased susceptibility to colitis induced by dextran sodium sulfate (DSS) and pulmonary fibrosis induced by bleomycin (Cay-13877).Synonyms: AUT-like 1 Cysteine Endopeptidase, Autophagin-1, Autophagy-related Protein 4 Homolog B, Cysteine Protease ATG4B, hAPG4B. Purity: >80% estimated by SDS-PAGE. Source: Recombinant N-terminal histidine-tagged ATG4B expressed in E. coli. Amino Acids: 2-393. MW: 43.4 kDa. Formulation: (Request formulation change), 50 mM HEPES, pH 8.0, 150 mM sodium chloride, 10% glycerol. UniProt Accession #: Q9Y4P1.