Tumor necrosis factor ligand superfamily member 15 (Tnfsf15), partial (Active), recombinant mouse

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 61-252aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: AGQLRVPGKD CMLRAITEER SEPSPQQVYS PPRGKPRAHL TIKKQTPAPH LKNQLSALHW EHDLGMAFTK NGMKYINKSL VIPESGDYFI YSQITFRGTT SVCGDISRGR RPNKPDSITV VITKVADSYP EPARLLTGSK SVCEISNNWF QSLYLGAMFS LEEGDRLMVN VSDISLVDYT KEDKTFFGAF LL. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Tnfsf15 at 2 µg/mL can bind Anti-TNFSF15 recombinant antibody (CSB-RA023992MA1HU). The EC50 is 1.671-2.506 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. Reference: TL1A is a TNF-like ligand for DR3 and TR6/DcR3 and functions as a T cell costimulator. Migone T.-S., Zhang J., Luo X., Zhuang L., Chen C., Hu B., Hong J.S., Perry J.W., Chen S.-F., Wei P. Immunity 16:479-492 (2002). Function: nan