Transforming growth factor beta-1 proprotein (Tgfb1), partial (Active), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 279-390aa. Protein Length: Partial. Tag Info: Tag-Free. Target Protein Sequence: ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASASPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/µg as determined by LAL method. Biological Activity: The ED50 as determined by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells is 5-25 pg/ml. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 4 mM HCl. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Transforming growth factor beta 1 (TGFbeta1) is the prototype of a growing superfamily of peptide growth factors and plays a prominent role in a variety of cellular processes, including cell-cycle progression, cell differentiation, reproductive function, development, motility, adhesion, neuronal growth, bone morphogenesis, wound healing, and immune surveillance. TGF-beta1, TGF-beta2 and TGF-beta3 signal via the same heteromeric receptor complex, consisting of a ligand binding TGF-beta receptor type II (TbetaR-II), and a TGF-beta receptor type I (TbetaR-I). Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF-beta expression is found in cartilage, bone, teeth, muscle, heart, blood vessels, haematopoitic cells, lung, kidney, gut, liver, eye, ear, skin, and the nervous system. Reference: n/a. Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts