Indian hedgehog protein (IHH), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 203-411aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: CFPAGAQVRL ESGARVALSA VRPGDRVLAM GEDGSPTFSD VLIFLDREPH RLRAFQVIET QDPPRRLALT PAHLLFTADN HTEPAARFRA TFASHVQPGQ YVLVAGVPGL QPARVAAVST HVALGAYAPL TKHGTLVVED VVASCFAAVA DHHLAQLAFW PLRLFHSLAW GSWTPGEGVH WYPQLLYRLG RLLLEEGSFH PLGMSGAGS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) . Reference: Expression of Sonic hedgehog and its receptor Patched/Smoothened in human cancer cell lines and embryonic organs.Tate G., Kishimoto K., Mitsuya T.J. Biochem. Mol. Biol. Biophys. 4:27-34(2000). Function: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification