Indian hedgehog protein (IHH), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: E.Coli. Expression Region: 29-202aa. Protein Length: Partial. Tag Info: Tag-Free. Target Protein Sequence: II+GPGRVVGSRR RPPRKLVPLA YKQFSPNVPE KTLGASGRYE GKIARSSERF KELTPNYNPD IIFKDEENTG ADRLMTQRCK DRLNSLAISV MNQWPGVKLR VTEGWDEDGH HSEESLHYEG RAVDITTSDR DRNKYGLLAR LAVEAGFDWV YYESKAHVHC SVKSEHSAAA KTGG. Purity: >96% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/µg as determined by LAL method. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 µg/ml. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 1 × PBS, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) (By similarity). {ECO:0000250}. Reference: n/a. Function: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification