Fibroblast growth factor 19 (FGF19), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: E.Coli. Expression Region: 23-216aa. Protein Length: Partial. Tag Info: Tag-Free. Target Protein Sequence: M+RPLAFSDAGP HVHYGWGDPI RLRHLYTSGP HGLSSCFLRI RADGVVDCAR GQSAHSLLEI KAVALRTVAI KGVHSVRYLC MGADGKMQGL LQYSEEDCAF EEEIRPDGYN VYRSEKHRLP VSLSSAKQRQ LYKNRGFLPL SHFLPMLPMV PEEPEDLRGH LESDMFSSPL ETDSMDPFGL VTGLEAVRSP SFEK. Purity: >95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/µg as determined by LAL method. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 x 10^3 IU/mg. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. {ECO:0000269, PubMed:12815072, ECO:0000269, PubMed:16597617, ECO:0000269, PubMed:17623664, ECO:0000269, PubMed:19085950}. Reference: n/a. Function: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4.