Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides . In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the... read more »
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Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

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GAPD, Human GAPDH, Real Time PCR Primer Set
GAPD, Human GAPDH, Real Time PCR Primer Set

Item number: VHPS-3541

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: GAPD, GAPDH, CDABP0047, EC=1.2.1.12, EC=2.6.99.-, Peptidyl-cysteine S-nitrosylase GAPDH, Glyceraldehyde-3-phosphate...
Application: RNA quantification
45.00€ *
Review
Human Housekeeping Genes Primer Sets
Human Housekeeping Genes Primer Sets

Item number: HHK-1

Includes primer sets for ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, RPL13A, TBP and TFRC. Normalize real time PCR results to multiple housekeepers rather than one for more accurate quantitation. Perform up to 1000 assays (based on 20 µl assay volume) for each housekeeping gene.
Application: RT-PCR, Gene expression analysis
145.00€ *
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ACTB, Human actin, beta, Real Time PCR Primer Set
ACTB, Human actin, beta, Real Time PCR Primer Set

Item number: VHPS-110

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: ACTB, Beta-actin, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
Application: RNA quantification
45.00€ *
Review
STI11 (Genital ulcer 2) (QuantStudio) Multiplexed qPCR Assay Bundle
STI11 (Genital ulcer 2) (QuantStudio) Multiplexed qPCR...

Item number: Cay41403-1

Oliogonucleotide recipes are optimized for QuantStudio 5, 6, 7, 12K. qPCR Enzyme, positive controls, and spike-in control are included. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme...
Keywords: Sexually Transmitted Infection
Application: Optimized for QuantStudio 5, 6, 7, 12K
814.00€ *
Review
Bacterial Vaginosis Primary (QuantStudio) Multiplexed qPCR Assay
Bacterial Vaginosis Primary (QuantStudio) Multiplexed...

Item number: Cay41401-1

Oliogonucleotide recipes are optimized for QuantStudio 5, 6, 7, 12K. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: Spike-in RPP30 control  Tube 4: 2X qPCR enzyme mastermix Organism Type: Bacteria Gram+, Multiplexed Reaction Panel:...
Application: Optimized for QuantStudio 5, 6, 7, 12K
814.00€ *
Review
Wound4 (Bio-Rad) Multiplexed qPCR Assay Bundle
Wound4 (Bio-Rad) Multiplexed qPCR Assay Bundle

Item number: Cay41488-1

Oliogonucleotide recipes are optimized for Bio-Rad CFX96 or CFX384. Includes 3 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: 2X qPCR enzyme mastermix Organism Type: Bacteria Gram-, Bacteria Gram+, Multiplexed Reaction Panels: Wound Colors:...
Keywords: WND4
Application: Optimized for Bio-Rad CFX96 / CFX384
814.00€ *
Review
Candidiasis Primary (QuantStudio) Multiplexed qPCR Assay Bundle
Candidiasis Primary (QuantStudio) Multiplexed qPCR Assay...

Item number: Cay41402-1

Oliogonucleotide recipes are optimized for QuantStudio 5, 6, 7, 12K. qPCR Enzyme, positive controls, and spike-in control are included. Includes 4 tubes:  Tube 1: 5X Primers and probes for 4 pathogens and human control  Tube 2: Synthetic positive controls  Tube 3: 2X qPCR enzyme mastermix  Tube 4: Spike-in RPP30...
Keywords: Clavispora lusitaniae
Application: Optimized for QuantStudio 5, 6, 7, 12K
814.00€ *
Review
E.coli Residual Total RNA Detection Kit
E.coli Residual Total RNA Detection Kit

Item number: ARG83092.100

E.coli Residual Total RNA Detection Kit is designed for the quantitative detection of residual E.coli total RNA in various biological products to improve control quality of nucleic acid. This kit adopts the principle of the RT-PCR fluorescent probe, combining reverse transcription PCR technology and fluorescent...
Application: qPCR
2,630.00€ *
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TDO Primer Mix-qPCR Assay
TDO Primer Mix-qPCR Assay

Item number: BPS-71271

Primer mix for amplifying human TDO cDNA (1221 bp) using dye-based, real time, quantitative PCR (RT-qPCR).
Keywords: TO, TDO, TRPO, Tryptophanase, Tryptophan pyrrolase, Tryptophan oxygenase, Tryptamin 2,3-dioxygenase, Tryptophan...
Application: RT-qPCR, RT-PCR
Species reactivity: human
257.00€ *
Review
IDO1 Primer Mix-qPCR Assay
IDO1 Primer Mix-qPCR Assay

Item number: BPS-71272

Primer mix for amplifying human IDO1 cDNA (1212 bp) using dye-based, real time, quantitative PCR (RT-qPCR).
Keywords: IDO, IDO-1, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase,
Application: RT-qPCR, RT-PCR
Species reactivity: human
257.00€ *
Review
COVID-19 (SARS-CoV-2) Triplex RT-qPCR Detection Kit
COVID-19 (SARS-CoV-2) Triplex RT-qPCR Detection Kit

Item number: G-VA0001.100

This product is a multiplex fluorescent probe-based RT-qPCR assay system for the detection of COVID-19, the disease caused by the novel coronavirus SARS-CoV-2. The Taqman fluorescent probe is a specific oligonucleotide based on a reporter-quencher mechanism. For each probe, the 5'-end is labeled with a fluorophore,...
Keywords: SARS-CoV-2 Detection Kit - Triplex Taqman RT-qPCR Kit for Coronavirus Detection (2019-nCoV)
Application: RT-qPCR
Species reactivity: human
954.00€ *
Review
COVID-19 (SARS-CoV-2) & Influenza A/B Multiplex QPCR Detection Kit
COVID-19 (SARS-CoV-2) & Influenza A/B Multiplex QPCR...

Item number: G-VA0002.100

This product is a multiplex reverse transcription quantitative real-time PCR (RT-qPCR) kit based on Taqman probe method. The 3' end of each detection probe is labeled with a quenching group, and the 5' end is labeled with a different reporter group. When the probe is intact, since the quenching group is close to the...
Application: RT-qPCR
Species reactivity: human
791.00€ *
Review
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