PLK4 (Vector pUC, Accession No. BC036023)

Length: 2913 Sequence: atggcgacct gcatcgggga gaagatcgag gattttaaag ttggaaatct gcttggtaaa ggatcatttg ctggtgtcta cagagctgag tccattcaca ctggtttgga agttgcaatc aaaatgatag ataagaaagc catgtacaaa gcaggaatgg tacagagagt ccaaaatgag gtgaaaatac attgccaatt gaaacatcct tctatcttgg agctttataa ctattttgaa gatagcaatt atgtgtatct ggtattagaa atgtgccata atggagaaat gaacaggtat ctaaagaata gagtgaaacc cttctcagaa aatgaagctc gacacttcat gcaccagatc atcacaggga tgttgtatct tcattctcat ggtatactac accgggacct cacactttct aacctcctac tgactcgtaa tatgaacatc aagattgctg attttgggct ggcaactcaa ctgaaaatgc cacatgaaaa gcactataca ttatgtggaa ctcctaacta catttcacca gaaattgcca ctcgaagtgc acatggcctt gaatctgatg tttggtccct gggctgtatg ttttatacat tacttatcgg gagaccaccc ttcgacactg acacagtcaa gaacacatta aataaagtag tattggcaga ttatgaaatg ccatcttttt tgtcaataga ggccaaggac cttattcacc agttacttcg tagaaatcca gcagatcgtt taagtctgtc ttcagtattg gaccatcctt ttatgtcccg aaattcttca acaaaaagta aagatttagg aactgtggaa gactcaattg atagtgggca tgccacaatt tctactgcaa ttacagcttc ttccagtacc agtataagtg gtagtttatt tgacaaaaga agacttttga ttggtcagcc actcccaaat aaaatgactg tatttccaaa gaataaaagt tcaactgatt tttcttcttc aggagatgga aacagttttt atactcagtg gggaaatcaa gaaaccagta atagtggaag gggaagagta attcaagatg cagaagaaag gccacattct cgataccttc gtagagctta ttcctctgat agatctggca cttctaatag tcagtctcaa gcaaaaacat atacaatgga acgatgtcac tcagcagaaa tgctttcagt gtccaaaaga tcaggaggag gtgaaaatga agagaggtac tcacccacag acaacaatgc caacattttt aacttcttta aagaaaagac atccagtagt tctggatctt ttgaaagacc tgataacaat caagcactct ccaatcatct ttgtccagga aaaactcctt ttccatttgc agacccgaca cctcagactg aaaccgtaca acagtggttt gggaatctgc aaataaatgc tcatttaaga aaaactactg aatatgacag catcagccca aaccgggact tccagggcca tccagatttg cagaaggaca catcaaaaaa tgcctggact gatacaaaag tcaaaaagaa ctctgatgct tctgataatg cacattctgt aaaacagcaa aataccatga aatatatgac tgcacttcac agtaaacctg agataatcca acaagaatgt gtttttggct cagatcctct ttctgaacag agcaagacta ggggtatgga gccaccatgg ggttatcaga atcgtacatt aagaagcatt acatctccgt tggttgctca caggttaaaa ccaatcagac agaaaaccaa aaaggctgtg gtgagcatac ttgattcaga ggaggtgtgt gtggagcttg taaaggagta tgcatctcaa gaatatgtga aagaagttct tcagatatct agtgatggaa atacgatcac tatttattat ccaaatggtg gtagaggttt tcctcttgct gatagaccac cctcacctac tgacaacatc agtaggtaca gctttgacaa tttaccagaa aaatactggc gaaaatatca atatgcttcc aggtttgtac agcttgtaag atctaaatct cccaaaatca cttattttac aagatatgct aaatgcattt tgatggagaa ttctcctggt gctgattttg aggtttggtt ttatgatggg gtaaaaatac acaaaacaga agatttcatt caggtgattg aaaagacagg gaagtcttac actttaaaaa gtgaaagtga agttaatagc ttgaaagagg agataaaaat gtatatggac catgctaatg agggtcatcg tatttgttta gcactggaat ccataatttc agaagaggaa aggaaaacta ggagtgctcc ctttttccca ataatcatag gaagaaaacc tggtagtact agttcaccta aggccttatc acctcctcct tctgtggatt caaattaccc aacgagagag agagcatctt tcaacagaat ggtcatgcat agtgctgctt ctccaacaca ggcaccaatc cttaatccct ctatggttac aaatgaagga cttggtctta caactacagc ttctggaaca gacatctctt ctaatagtct aaaagattgt cttcctaaat cagcacaact tttgaaatct gtttttgtga aaaatgttgg ttgggctaca cagttaacta gtggagctgt gtgggttcag tttaatgatg ggtcccagtt ggttgtgcag gcaggagtgt cttctatcag ttatacctca ccaaatggtc aaacaactag gtatggagaa aatgaaaaat taccagacta catcaaacag aaattacagt gtctgtcttc catccttttg atgttttcta atccgactcc taattttcat Protein function: Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (PubMed:22020124). [The UniProt Consortium]