LYPLA1 (Vector pENTR223.1, Accession No. BC008652)

Length: 693 Sequence: atgtgcggca ataacatgtc aaccccgctg cccgccatcg tgcccgccgc ccggaaggcc accgctgcgg tgattttcct gcatggattg ggagatactg ggcacggatg ggcagaagcc tttgcaggta tcagaagttc acatatcaaa tatatctgcc cgcatgcgcc tgttaggcct gttacattaa atatgaacgt ggctatgcct tcatggtttg atattattgg gctttcacca gattcacagg aggatgaatc tgggattaaa caggcagcag aaaatataaa agctttgatt gatcaagaag tgaagaatgg cattccttct aacagaatta ttttgggagg gttttctcag ggaggagctt tatctttata tactgccctt accacacagc agaaactggc aggtgtcact gcactcagtt gctggcttcc acttcgggct tcctttccac agggtcctat cggtggtgct aatagagata tttctattct ccagtgccac ggggattgtg accctttggt tcccctgatg tttggttctc ttacggtgga aaaactaaaa acattggtga atccagccaa tgtgaccttt aaaacctatg aaggtatgat gcacagttcg tgtcaacagg aaatgatgga tgtcaagcaa ttcattgata aactcctacc tccaattgat Protein function: Acts as a acyl-protein thioesterase (PubMed:19439193, PubMed:20418879). Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS (PubMed:20418879). Has depalmitoylating activity toward KCNMA1 (PubMed:22399288). Could also depalmitoylate ADRB2 (PubMed:27481942). Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC) (PubMed:19439193). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso- PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity (PubMed:19439193). Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA (PubMed:21393252). [The UniProt Consortium]