KSR2 (Vector pUC, Accession No. BC127603)

Length: 2490 Sequence: ATGACGGATG AACAGGTGTG CGAGACTGTG GAGAAATACG GAGCCAACCG GGAGGAGTGT GCCCGCCTCA ACGCCTCCCT CTCCTGCCTC AGGAATGTCC ACATGTCAGG AGGCAACCTT TCCAAACAAG ACTGGACCAT CCAGTGGCCC ACCACAGAGA CGGGGAAGGA GAACAATCCC GTGTGCCCCC CGGAGCCCAC CCCGTGGATC CGCACCCATC TCTCCCAGAG CCCCAGGGTC CCGTCCAAGT GCGTCCAGCA CTATTGTCAC ACCAGCCCCA CTCCCGGGGC CCCTGTGTAC ACCCACGTGG ACAGGCTTAC CGTGGACGCC TACCCGGGCT TGTGCCCGCC CCCGCCACTG GAGTCGGGCC ACCGTTCCCT GCCCCCATCG CCCCGGCAGC GGCACGCGGT CCGCACCCCG CCGCGCACCC CCAACATCGT CACCACCGTG ACCCCGCCGG GCACGCCGCC CATGAGGAAG AAGAACAAGC TGAAGCCCCC GGGGACCCCA CCGCCCTCCT CCCGAAAACT GATACACTTG ATCCCGGGAT TCACCGCGCT GCATCGGAGC AAATCCCACG AGTTCCAGCT GGGGCACCGC GTGGACGAGG CCCACACGCC CAAAGCCAAG AAGAAGAGCA AACCCTTGAA CCTCAAGATC CACAGCAGCG TAGGCAGCTG CGAGAACATC CCCTCTCAGC AGCGCTCCCC GCTGCTGTCC GAGCGCTCCC TCCGCTCCTT CTTTGTGGGA CACGCACCTT TCCTGCCTTC CACCCCTCCT GTTCACACTG AGGCCAACTT CTCTGCAAAC ACACTGTCAG TGCCACGCTG GTCCCCGCAG ATCCCTCGCA GAGATCTTGG CAACTCCATC AAGCACAGGT TTTCCACCAA GTACTGGATG TCTCAGACGT GCACAGTCTG TGGGAAAGGG ATGCTTTTTG GCCTCAAGTG TAAAAACTGC AAGTTAAAGT GCCACAACAA ATGCACCAAA GAAGCCCCAC CCTGTCATCT TCTGATCATC CACCGAGGAG ATCCAGCAAG GTTAGTCCGG ACAGAGTCCG TTCCGTGTGA CATCAACAAC CCTCTACGGA AGCCACCTCG CTATTCAGAC CTGCACATCA GTCAGACGCT CCCCAAAACC AACAAAATCA ACAAGGACCA CATCCCTGTC CCTTACCAGC CAGACTCCAG CAGCAACCCC TCCTCCACGA CGTCCTCCAC GCCCTCCTCG CCAGCACCCC CCCTCCCTCC TAGTGCCACG CCGCCTTCTC CCCTACACCC TTCCCCACAG TGCACACGGC AGCAGAAGAA CTTCAACCTG CCAGCATCCC ACTACTACAA ATACAAGCAG CAGTTCATCT TCCCAGATGT GGTGCCGGTG CCGGAGACGC CGACCCGGGC GCCCCAGGTC ATCCTGCATC CGGTGACCTC GAATCCAATC TTGGAAGGAA ATCCATTACT TCAAATTGAA GTGGAGCCAA CGTCGGAGAA TGAAGAGGTC CATGATGAGG CCGAAGAGTC AGAGGATGAC TTCGAGGAGA TGAACCTGTC CCTCCTCTCG GCCCGGAGCT TCCCACGCAA GGCCAGCCAG ACCAGCATCT TCCTTCAGGA GTGGGACATC CCCTTTGAGC AGCTGGAGAT CGGCGAGCTC ATTGGAAAGG GCCGCTTTGG GCAAGTGTAC CACGGCCGCT GGCATGGCGA GGTGGCCATC CGGCTGATTG ACATTGAGAG GGACAACGAG GACCAGCTCA AGGCCTTCAA GCGGGAGGTG ATGGCCTACA GGCAGACACG GCATGAGAAC GTGGTGCTTT TCATGGGTGC CTGCATGAGC CCGCCTCACC TGGCCATCAT CACCAGCCTC TGTAAGGGAC GGACGCTCTA TTCCGTTGTG AGGGATGCCA AAATCGTTTT GGATGTCAAC AAAACCAGGC AGATTGCTCA AGAAATTGTG AAGGGCATGG GCTACCTCCA CGCCAAGGGA ATCCTACACA AGGACCTCAA GTCAAAGAAC GTCTTCTATG ACAACGGCAA AGTGGTCATC ACGGACTTTG GACTCTTCAG CATTTCTGGG GTGCTGCAGG CTGGCAGGCG GGAGGACAAA CTGCGCATCC AGAATGGCTG GCTATGCCAC CTGGCACCAG AGATCATCCG CCAGCTGTCC CCCGACACAG AGGAGGATAA GCTCCCCTTC TCCAAGCACT CTGACGTCTT TGCCCTTGGC ACAATCTGGT ATGAACTCCA CGCCAGGGAA TGGCCTTTCA AGACCCAACC AGCAGAGGCA ATAATCTGGC AAATGGGCAC AGGCATGAAA CCCAACCTCA GCCAGATTGG CATGGGAAAA GAAATCTCGG ACATTCTTCT CTTCTGCTGG GCCTTTGAAC AAGAAGAGAG ACCTACCTTC ACCAAGCTCA TGGACATGCT GGAGAAACTG CCAAAGCGAA ACCGTCGCCT GTCTCACCCT GGACATTTCT GGAAGTCTGC Protein function: Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF, upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 (PubMed:29433126). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF- kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production. [The UniProt Consortium]