DYRK3 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 1707 Sequence: atgaagtgga aagagaagtt gggggatggt gtctatgaca ccttcatgat gatagatgaa accaaatgtc ccccctgttc aaatgtactc tgcaatcctt ctgaaccacc tccacccaga agactaaata tgaccactga gcagtttaca ggagatcata ctcagcactt tttggatgga ggtgagatga aggtagaaca gctgtttcaa gaatttggca acagaaaatc caatactatt cagtcagatg gcatcagtga ctctgaaaaa tgctctccta ctgtttctca gggtaaaagt tcagattgct tgaatacagt aaaatccaac agttcatcca aggcacccaa agtggtgcct ctgactccag aacaagccct gaagcaatat aaacaccacc tcactgccta tgagaaactg gaaataatta attatccaga aatttacttt gtaggtccaa atgccaagaa aagacatgga gttattggtg gtcccaataa tggagggtat gatgatgcag atggggccta tattcatgta cctcgagacc atctagctta tcgatatgag gtgctgaaaa ttattggcaa ggggagtttt gggcaggtgg ccagggtcta tgatcacaaa cttcgacagt acgtggccct aaaaatggtg cgcaatgaga agcgctttca tcgtcaagca gctgaggaga tccggatttt ggagcatctt aagaaacagg ataaaactgg tagtatgaac gttatccaca tgctggaaag tttcacattc cggaaccatg tttgcatggc ctttgaattg ctgagcatag acctttatga gctgattaaa aaaaataagt ttcagggttt tagcgtccag ttggtacaca agtttgccca gtccatcttg caatctttgg atgccctcca caaaaataag attattcact gcgatctgaa gccagaaaac attctcctga aacaccacgg gcgcagttca accaaggtca ttgactttgg gtccagctgt ttcgagtacc agaagctcta cacatatatc cagtctcggt tctacagagc tccagaaatc atcttaggaa gccgctacag cacaccaatt gacatatgga gttttggctg catccttgca gaacttttaa caggacagcc tctcttccct ggagaggatg aaggagacca gttggcctgc atgatggagc ttctagggat gccaccacca aaacttctgg agcaatccaa acgtgccaag tactttatta attccaaggg cataccccgc tactgctctg tgactaccca ggcagatggg agggttgtgc ttgtgggggg tcgctcacgt aggggtaaaa agcggggtcc cccaggcagc aaagactggg ggacagcact gaaagggtgt gatgactact tgtttataga gttcttgaaa aggtgtcttc actgggaccc ctctgcccgc ttgaccccag ctcaagcatt aagacaccct tggattagca agtctgtccc cagacctctc accaccatag acaaggtgtc agggaaacgg gtagttaatc ctgcaagtgc tttccaggga ttgggttcta agctgcctcc agttgttgga atagccaata agcttaaagc taacttaatg tcagaaacca atggtagtat acccctatgc agtgtattgc caaaactgat Protein function: Dual-specificity protein kinase that promotes disassembly of several types of membraneless organelles during mitosis, such as stress granules, nuclear speckles and pericentriolar material (PubMed:29973724). Dual-specificity tyrosine-regulated kinases (DYRKs) autophosphorylate a critical tyrosine residue in their activation loop and phosphorylate their substrate on serine and threonine residues (PubMed:9748265, PubMed:29634919). Acts as a central dissolvase of membraneless organelles during the G2-to-M transition, after the nuclear-envelope breakdown: acts by mediating phosphorylation of multiple serine and threonine residues in unstructured domains of proteins, such as SRRM1 and PCM1 (PubMed:29973724). Does not mediate disassembly of all membraneless organelles: disassembly of P-body and nucleolus is not regulated by DYRK3 (PubMed:29973724). Dissolution of membraneless organelles at the onset of mitosis is also required to release mitotic regulators, such as ZNF207, from liquid-unmixed organelles where they are sequestered and keep them dissolved during mitosis (PubMed:29973724). Regulates mTORC1 by mediating the dissolution of stress granules: during stressful conditions, DYRK3 partitions from the cytosol to the stress granule, together with mTORC1 components, which prevents mTORC1 signaling (PubMed:23415227). When stress signals are gone, the kinase activity of DYRK3 is required for the dissolution of stress granule and mTORC1 relocation to the cytosol: acts by mediating the phosphorylation of the mTORC1 inhibitor AKT1S1, allowing full reactivation of mTORC1 signaling (PubMed:23415227). Also acts as a negative regulator of EPO-dependent erythropoiesis: may place an upper limit on red cell production during stress erythropoiesis (PubMed:10779429). Inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells (PubMed:10779429). Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1: this in turn inhibits p53/TP53 activity and apoptosis (PubMed:20167603). [The UniProt Consortium]