CYP2C9 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 489 Sequence: atggattctc ttgtggtcct tgtgctctgt ctctcatgtt tgcttctcct ttcactctgg agacagagct ctgggagagg aaaactccct cctggcccca ctcctctccc agtgattgga aatatcctac agataggtat taaggacatc agcaaatcct taaccaatct ctcaaaggtc tatggccctg tgttcactct gtattttggc ctgaaaccca tagtggtgct gcatggatat gaagcagtga aggaagccct gattgatctt ggagaggagt tttctggaag aggcattttc ccactggctg aaagagctaa cagaggattt ggaattgttt tcagcaatgg aaagaaatgg aaggagatcc ggcgtttctc cctcatgacg ctgcggaatt ttgggatggg gaagaggagc attgaggacc gtgttcaaga ggaagcccgc tgccttgtgg aggagttgag aaaaaccaag Protein function: A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids (PubMed:7574697, PubMed:9866708, PubMed:9435160, PubMed:12865317, PubMed:15766564, PubMed:19965576, PubMed:21576599). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:7574697, PubMed:9866708, PubMed:9435160, PubMed:12865317, PubMed:15766564, PubMed:19965576, PubMed:21576599). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA) (PubMed:7574697, PubMed:15766564, PubMed:19965576, PubMed:9866708). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed:21576599). Exhibits low catalytic activity for the formation of catechol estrogens from 17beta- estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2 (PubMed:12865317). Catalyzes bisallylic hydroxylation and hydroxylation with double-bond migration of polyunsaturated fatty acids (PUFA) (PubMed:9866708, PubMed:9435160). Also metabolizes plant monoterpenes such as limonene. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol (PubMed:11950794). Contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S- warfarin, diclofenac, phenytoin, tolbutamide and losartan (PubMed:25994031). [The UniProt Consortium]