CUL1 (Vector pUC, Accession No. BC125119)

Length: 2331 Sequence: ATGTCGTCAA CCCGGAGCCA GAACCCCCAC GGCCTGAAGC AGATTGGCCT GGACCAGATC TGGGACGACC TCAGAGCCGG CATCCAGCAG GTGTACACAC GGCAGAGCAT GGCCAAGTCC AGATATATGG AGCTCTACAC TCATGTTTAT AACTACTGTA CTAGTGTTCA CCAGTCAAAC CAAGCACGAG GAGCTGGAGT TCCTCCTTCT AAGTCGAAAA AGGGGCAGAC ACCTGGAGGA GCTCAGTTTG TTGGCCTGGA ATTATATAAA CGACTTAAGG AATTTTTGAA GAATTACTTG ACAAATCTTC TTAAGGATGG AGAAGATTTG ATGGATGAGA GTGTACTGAA ATTCTACACT CAACAATGGG AAGATTATCG ATTTTCAAGC AAAGTGCTGA ATGGAATTTG TGCCTACCTC AATAGACATT GGGTTCGCCG TGAATGTGAC GAAGGACGAA AAGGAATATA TGAAATCTAT TCGCTTGCAT TGGTGACTTG GAGAGACTGT CTGTTCAGGC CACTGAATAA ACAGGTAACA AATGCTGTTT TAAAGCTGAT TGAAAAGGAA AGGAATGGTG AAACCATCAA TACAAGATTG ATTAGTGGAG TTGTACAGTC TTACGTGGAA TTGGGGCTGA ATGAAGATGA TGCATTTGCA AAGGGCCCTA CGTTAACAGT GTATAAAGAA TCCTTTGAAT CTCAATTTTT GGCTGACACA GAGAGATTTT ATACCAGAGA GAGTACTGAA TTCTTGCAGC AGAACCCAGT TACTGAATAT ATGAAAAAGG CAGAGGCTCG TCTGCTTGAG GAACAACGAA GAGTTCAGGT TTACCTTCAT GAAAGCACAC AAGATGAATT AGCAAGGAAA TGTGAACAAG TCCTCATTGA AAAACACTTG GAAATTTTCC ACACAGAATT TCAGAATTTA TTGGATGCTG ACAAAAATGA AGATTTGGGA CGCATGTATA ATCTTGTATC TAGAATCCAG GATGGCCTAG GAGAATTGAA AAAACTGTTG GAGACACACA TTCATAATCA GGGTCTTGCA GCCATTGAAA AGTGTGGAGA AGCTGCTTTA AATGACCCCA AAATGTATGT ACAGACAGTG CTTGATGTTC ATAAAAAATA CAATGCCCTG GTAATGTCTG CATTCAACAA TGACGCTGGC TTTGTGGCTG CTCTTGATAA GGCTTGTGGT CGCTTCATAA ACAACAACGC GGTTACCAAG ATGGCCCAAT CATCCAGTAA ATCCCCTGAG TTGCTGGCTC GATACTGTGA CTCCTTGTTG AAGAAAAGTT CCAAGAACCC AGAGGAGGCA GAACTAGAAG ACACACTCAA TCAAGTGATG GTTGTCTTCA AGTACATAGA AGACAAAGAC GTATTTCAGA AGTTCTATGC GAAGATGCTC GCCAAGAGGC TCGTCCACCA GAACAGTGCA AGTGACGATG CCGAAGCCAG CATGATCTCC AAGTTAAAGC AAGCTTGCGG GTTCGAGTAC ACCTCTAAAC TTCAGCGCAT GTTTCAAGAC ATTGGCGTGA GCAAAGATCT GAACGAGCAA TTCAAAAAGC ACTTGACAAA CTCAGAACCC CTAGACTTGG ATTTCAGCAT TCAAGTGCTG AGCTCCGGGT CCTGGCCCTT CCAGCAGTCT TGTACATTTG CCTTGCCGTC AGAGTTGGAA CGTAGTTATC AGCGATTCAC AGCTTTCTAC GCCAGCCGCC ACAGTGGCCG AAAATTGACG TGGTTATATC AGTTGTCTAA AGGAGAATTG GTAACTAACT GCTTCAAAAA CAGATATACT TTGCAGGCGT CGACATTCCA GATGGCTATC CTGCTTCAGT ACAACACGGA AGATGCCTAC ACTGTGCAGC AGCTGACCGA CAGCACTCAA ATTAAAATGG ACATTTTGGC GCAAGTTTTA CAGATTTTAT TAAAGTCGAA GCTATTGGTC TTGGAAGATG AAAATGCAAA TGTTGATGAG GTGGAATTGA AGCCAGATAC CTTAATAAAA TTATATCTTG GTTATAAAAA TAAGAAATTA AGGGTTAACA TCAATGTGCC AATGAAAACC GAACAGAAGC AGGAACAAGA AACCACACAC AAAAACATCG AGGAAGACCG CAAACTACTG ATTCAGGCGG CCATCGTGAG AATCATGAAG ATGAGGAAGG TTCTGAAACA CCAGCAGTTA CTTGGCGAGG TCCTCACTCA GCTGTCCTCC AGGTTCAAAC CTCGAGTCCC TGTGATCAAG AAATGCATTG ACATTCTAAT TGAGAAAGAA TATTTGGAGC GAGTGGATGG TGAAAAGGAC ACCTACAGTT ACTTGGCTTA Protein function: Core component of multiple cullin-RING-based SCF (SKP1-CUL1- F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). In the SCF complex, serves as a rigid scaffold that organizes the SKP1- F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68 (PubMed:25704143, PubMed:25503564). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of CCNE1, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys- 22', the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2. [The UniProt Consortium]