ALKBH3 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 513 Sequence: atggaggaaa aaagacggcg agcccgagtt cagggagcct gggctgcccc tgttaaaagc caggccattg ctcagccagc taccactgct aagagccatc tccaccagaa gcctggccag acctggaaga acaaagagca tcatctctct gacagagagt ttgtgttcaa agaacctcag caggtagtac gtagagctcc tgagccacga gtgattgaag agggtgtgta tgaaatcagc ctgtcaccca caggtgtatc tagggtctgt ttgtatcctg gctttgttga cgtgaaagaa gctgactgga tattggaaca gctttgtcaa gatgttccct ggaaacagag gaccggcatc agagaggatt ccatattgca actaaccttt aagaagtcag cacctgttag tggaaccgcg actgctccgc agagctgctg gtatgagcgc ccgtcgccac cccacatccc aggcccagcc attctgacaa gaactagact ttgggctcct Protein function: Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A) (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation (PubMed:12486230, PubMed:12594517, PubMed:16174769, PubMed:25944111). Has a strong preference for single- stranded DNA (PubMed:12486230, PubMed:12594517, PubMed:16174769). Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3 (PubMed:22055184). Also acts on RNA (PubMed:12594517, PubMed:16174769, PubMed:26863196, PubMed:26863410, PubMed:16858410). Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons (PubMed:26863196, PubMed:26863410). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:22055184, PubMed:16858410). [The UniProt Consortium]