Lipid 331

Lipid 331 is a biodegradable cyclic ionizable cationic lipid (pKa = 7.53) that has been used in the formation of lipid nanoparticles (LNPs) for the delivery of mRNA in vitro and in vivo. LNPs containing lipid 331 and encapsulating 5-methoxyuridine-modified mRNA encoding ovalbumin induce IFN regulatory factor (IRF) activation in a reporter assay using THP-1 monocytes. Intranasal administration of LNPs containing lipid 331 and DOTAP (Cay-15110, Cay-38905) and encapsulating mRNA encoding a fusion of the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein, also known as surface glycoprotein, with mouse complement C3d as an adjuvant increase serum anti-SARS-CoV-2 spike glycoprotein RBD IgG titers in mice.Formal Name: di((9Z,12Z)-octadeca-9,12-dien-1-yl) 4,4'-(2-(tert-butoxycarbonyl)-1-(2-(2-ethylpiperidin-1-yl)ethyl)-2,5-dihydro-1H-imidazole-5,5-diyl)dibutyrate. Molecular Formula: C61H107N3O6. Formula Weight: 978.5. Purity: >95%. Formulation: (Request formulation change), A solution in ethanol. Solubility: Chloroform: Soluble, Methanol: Soluble. SMILES: O=C(OCCCCCCCC/C=C\C/C=C\CCCCC)CCCC1(CCCC(OCCCCCCCC/C=C\C/C=C\CCCCC)=O)C=NC(C(OC(C)(C)C)=O)N1CCN2CCCCC2CC. InChi Code: InChI=1S/C61H107N3O6/c1-7-10-12-14-16-18-20-22-24-26-28-30-32-34-36-40-52-68-56(65)45-42-47-61(54-62-58(59(67)70-60(4,5)6)64(61)51-50-63-49-39-38-44-55(63)9-3)48-43-46-57(66)69-53-41-37-35-33-31-29-27-25-23-21-19-17-15-13-11-8-2/h16-19,22-25,54-55,58H,7-15,20-21,26-53H2,1-6H3/b18-16-,19-17-,24-22-,25-23-. InChi Key: UGSRIRFHGLQYBQ-HBZIWDQGSA-N.