Cholic Acid-d4 MaxSpec(R) Standard

Cholic acid-d4 is intended for use as an internal standard for the quantification of cholic acid (Cay-20250) by GC- or LC-MS. Cholic acid is a primary bile acid. It is formed from cholesterol via a multistep process catalyzed by the cytochrome P450 (CYP) isoforms CYP7A1, CYP8B1, and CYP27A1. Cholic acid is conjugated to glycine or taurine by bile acid-CoA:amino acid N-acyltransferase (BAAT) to produce glycocholic acid (GCA, Cay-20276) and taurocholic acid (TCA, Cay-16215), respectively, in the liver, and is transformed into the secondary bile acid deoxycholic acid (DCA, Cay-20756) by intestinal microbiota. It induces C. difficile colony formation in an agar dilution assay when used at a concentration of 0.1% w/v. Dietary administration of cholic acid (0.4% w/w) increases serum cholesterol levels, biliary phospholipid secretion, and fecal DCA levels in rats. Cholic acid-d4 MaxSpec(R) standard is a quantitative grade standard of cholic acid-d4 (Cay-20849) that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This cholic acid4 MaxSpec(R) standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.Formal Name: (3alpha,5beta,7alpha,12alpha)-3,7,12-trihydroxy-cholan-24-oic-2,2,4,4-d4 acid. CAS Number: 116380-66-6. Synonyms: Cholalic Acid-d4, Cholalin-d4. Molecular Formula: C24H36D4O5. Formula Weight: 412.6. Purity: >95%. Formulation: (Request formulation change), A solution in methanol at 100 µg/ml. SMILES: O[C@@H]1C([2H])([2H])C[C@@]2(C)[C@@](C[C@@H](O)[C@]3([H])[C@]2([H])C[C@H](O)[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCC(O)=O)([H])C1([2H])[2H]. InChi Code: InChI=1S/C24H40O5/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29)/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-/m1/s1/i8D2,10D2. InChi Key: BHQCQFFYRZLCQQ-YFEOEUIKSA-N.