BODIPY 480/508-Cholesterol

BODIPY 480/508-cholesterol is a biologically active and cell-permeable analog of cholesterol that is tagged with a fluorescent BODIPY group at carbon 24. It co-localizes with dehydroergosterol, a marker of cholesterol, in HeLa cells and is trafficked from the plasma membrane to the endocytic recycling compartment in BHK cells. BODIPY 480/508-cholesterol displays excitation/emission maxima of 480/508 nm, respectively, and has been used to monitor sterol uptake and inter-organelle sterol flux in cells.Formal Name: (T-4)-[(3beta)-24-(3,5-dimethyl-1H-pyrrol-2-yl-kappaN)-24-(3,5-dimethyl-2H-pyrrol-2-ylidene-kappaN)chol-5-en-3-olato]difluoro-boron. CAS Number: 878557-19-8. Synonyms: BCh2. Molecular Formula: C36H51BF2N2O. Formula Weight: 576.6. Purity: >98%. Emission: 508 nm. Excitation: 480 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 1 mg/ml, DMSO: 1 mg/ml, DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml, Ethanol: 0.5 mg/ml. lambdamax: 243, 306, 496 nm. SMILES: O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCC(C5=C(C)C=C(C)N5[B-]6(F)F)=C7[N+]6=C(C)C=C7C. InChi Code: InChI=1S/C36H51BF2N2O/c1-21(8-10-29-33-22(2)18-24(4)40(33)37(38,39)41-25(5)19-23(3)34(29)41)30-12-13-31-28-11-9-26-20-27(42)14-16-35(26,6)32(28)15-17-36(30,31)7/h9,18-19,21,27-28,30-32,42H,8,10-17,20H2,1-7H3/t21-,27+,28+,30-,31+,32+,35+,36-/m1/s1. InChi Key: CFFWELDLDWZNRO-ZVEUZXBYSA-N.