Protonex(TM) Green 500-PEG12, SE

Extinction coefficient (cm^-1 M^-1): 4000. Excitation (nm): 445. Emission (nm): 503.The amino-reactive Protonex(TM) Green 500 SE (#21216) was developed for preparing Protonex(TM) Green 500 conjugates as fluorescent pH probes. However, the utility of Protonex(TM) Green 500 SE was limited by its poor solubility. Protonex(TM) Green 500-PEG12 SE (#21219) has been developed to significantly improve the poor solubility of Protonex(TM) Green 500 SE (#21216) while maintaining the excellent spectral and pH properties of Protonex(TM) Green 500. They have identical spectra and pH profile while Green 500-PEG12 has significantly improved solubility. Protonex(TM) Green 500 dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex(TM) Green 500 dye, making it an excellent acidotropic fluorescent probe. The fluorescence of Protonex(TM) Green 500 dye increases as pH decreases from neutral to the acidic. The lack of fluorescence outside the cell eliminates the wash steps. Protonex(TM) Green dye provides a powerful tool to monitor acidic cell compartments such as endosomes and lysosomes. Protonex(TM) Green dye is non-fluorescent outside the cells but fluoresces brightly green in acidic compartments (such as phagosomes, lysosomes and endosomes). This Protonex(TM) Green enables the specific detection of cellular acidic compartments with reduced signal variability and improved accuracy for imaging or flow applications. Protonex(TM) Green has the spectral properties similar to those of FITC, making the common filter set of FITC readily available to the assays of Protonex(TM) Green.