Fluorescein-5-maleimide

Fluorescein-5-maleimide is an activated fluorescent molecule (excitation: 494 nm, emission: 519 nm) that is used to easily conjugate fluorescein to proteins. The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond. The compound can be used to label proteins for the detection of conformational changes, assembly of multisubunit complexes, and ligand-binding processes.Formal Name: 1-(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-1H-pyrrole-2,5-dione. CAS Number: 75350-46-8. Synonyms: 5-Maleimido-fluorescein, 5-MF. Molecular Formula: C24H13NO7. Formula Weight: 427.4. Purity: >90%. Emission: 519 nm. Excitation: 494 nm. Formulation: (Request formulation change), A solid. Solubility: DMF: 30 mg/ml, DMSO: 20 mg/ml, Ethanol: 2 mg/ml. lambdamax: 225, 278 nm. SMILES: OC1=CC2=C(C=C1)C3(C(C=CC(N4C(C=CC4=O)=O)=C5)=C5C(O3)=O)C6=CC=C(O)C=C6O2. InChi Code: InChI=1S/C24H13NO7/c26-13-2-5-17-19(10-13)31-20-11-14(27)3-6-18(20)24(17)16-4-1-12(9-15(16)23(30)32-24)25-21(28)7-8-22(25)29/h1-11,26-27H. InChi Key: AYDAHOIUHVUJHQ-UHFFFAOYSA-N.